Influence of Populus genotype on gene expression by the wood decay fungus Phanerochaete chrysosporium.

نویسندگان

  • Jill Gaskell
  • Amber Marty
  • Michael Mozuch
  • Philip J Kersten
  • Sandra Splinter BonDurant
  • Grzegorz Sabat
  • Ali Azarpira
  • John Ralph
  • Oleksandr Skyba
  • Shawn D Mansfield
  • Robert A Blanchette
  • Dan Cullen
چکیده

We examined gene expression patterns in the lignin-degrading fungus Phanerochaete chrysosporium when it colonizes hybrid poplar (Populus alba × tremula) and syringyl (S)-rich transgenic derivatives. A combination of microarrays and liquid chromatography-tandem mass spectrometry (LC-MS/MS) allowed detection of a total of 9,959 transcripts and 793 proteins. Comparisons of P. chrysosporium transcript abundance in medium containing poplar or glucose as a sole carbon source showed 113 regulated genes, 11 of which were significantly higher (>2-fold, P < 0.05) in transgenic line 64 relative to the parental line. Possibly related to the very large amounts of syringyl (S) units in this transgenic tree (94 mol% S), several oxidoreductases were among the upregulated genes. Peptides corresponding to a total of 18 oxidoreductases were identified in medium consisting of biomass from line 64 or 82 (85 mol% S) but not in the parental clone (65 mol% S). These results demonstrate that P. chrysosporium gene expression patterns are substantially influenced by lignin composition.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 80 18  شماره 

صفحات  -

تاریخ انتشار 2014